Campos R; Peralta ME; Bearden DW; Miller WB 1994

• Authors: Campos R; Peralta ME; Bearden DW; Miller WB
• Title: Extraction and identification of mannitol from Antirrhinum majus l. leaves.
• Location: American society of horticultural science southern region 54th annual meeting, nashville, tennessee, usa, february 5-8, 1994. Hortscience, 29 (7). 1994. 738.
• Abstract: Soluble carbohydrate extracts from Antirrhinum majus L. leaves were fractionated by ion exchange chromatography. Putative mannitol was tentatively identified by retention behavior on two high performance liquid chromatography columns. Mannitol was confirmed using ¹H and ¹³C nuclear magnetic resonance (NMR), and by gas chromatography (GC) and mass spectroscopy (MS). The melting point of authentic and putative mannitol, and a 1:1 mix was from 164 to 166^oC. Using the EDTA-phloem exudate technique, mannitol was detected in phloem tissue associated with mature flowers, flower buds, and mature leaves, suggesting, that mannitol is translocated in Antirrhinum.