Davies B; Egeacortines M et al 1996

• Authors: Davies B; Egeacortines M; Silva ED; Saedler H; Sommer H
• Title: MULTIPLE INTERACTIONS AMONGST FLORAL HOMEOTIC MADS BOX PROTEINS
• Location: EMBO Journal 1996 Aug 15;15(16):4330-4343
• Abstract: Most known floral homeotic genes belong to the MADS box family and their products act in combination to specify floral organ identity by an unknown mechanism. We have used a yeast two-hybrid system to investigate the network of interactions between the Antirrhinum organ identity gene products, Selective heterodimerization is observed between MADS box factors, Exclusive interactions are detected between two factors, DEFICIENS (DEF) and GLOBOSA (GLO), previously known to heterodimerize and control development of petals and stamens, In contrast, a third fatter, PLENA (PLE), which is required for reproductive organ development, can interact with the products of MADS box genes ex-pressed at early, intermediate and late stages. We also demonstrate that heterodimerization of DEF and GLO requires the K box, a domain not found in non-plant MADS box factors, indicating that the plant MADS box factors mag have different criteria for interaction, The association of PLENA and the temporally intermediate MADS box factors suggests that part of their function in mediating between the meristem and organ identity genes is accomplished through direct interaction, These data reveal an unexpectedly complex network of interactions between the factors controlling flower development and have implications for the determination of organ identity. [References: 50]