Davies km; King Ga 1993

• Authors: Davies km; King Ga
• Title: Isolation and characterisation of asparagus-officinalis l. cdna clones encoding two forms of ubiquitin mrna
• Location: New zealand journal of crop and horticultural science, 21 (2). 1993. 153-159.
• Abstract: a cdna library constructed from the immature, rapidly developing tip region of spears of asparagus officinalis plants was screened by hybridisation with a cdna clone for a polyubiquitin mrna from Antirrhinum majus. two independent, positively hybridising cdnas were obtained, and their nucleotide sequence determined. one clone (ptip22) encodes a partial cdna for a ubiquitin-ribosomal fusion mrna and the other (ptip24) a partial cdna for a polyubiquitin mrna. the abundance of transcripts corresponding to ptip24 was relatively constant in a range of intact tissues but decreased following spear harvest. investigations of mrna abundance during spear development demonstrated that the changes detected were harvest-induced. transcripts corresponding to ptip22 were associated with regions of active cell development, and decreased in abundance following spear harvest.