Feinbaum RL et al. 1988
- Authors:
Feinbaum RL. Ausubel FM.
- Title:
Transcriptional regulation of the Arabidopsis thaliana chalcone synthase
gene.
- Reference location:
Molecular & Cellular Biology. 8(5):1985-92, 1988 May.
- Abstract:
We have cloned an Arabidopsis thaliana chalcone synthase (CHS) gene on the
basis of cross-hybridization with a Petroselinum hortense CHS cDNA clone.
The protein sequence deduced from the A. thaliana CHS DNA sequence is at
least 85% homologous to the CHS sequences from P. hortense, Antirrhinum
majus, and Petunia hybrida. Southern blot analysis indicated that CHS is a
single-copy gene in A. thaliana. High-intensity light treatment of A.
thaliana plants for 24 h caused a 50-fold increase in CHS enzyme activity
and an accumulation of visibly detectable levels of anthocyanin pigments
in the vegetative structures of these plants. A corresponding increase in
the steady-state level of CHS mRNA was detected after high-intensity light
treatment for the same period of time. The accumulation of CHS mRNA in
response to high-intensity light was due, at least in part, to an
increased rate of transcription of the CHS gene as demonstrated by nuclear
runoff experiments.
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