Hu hy; Chen Lh; Sung Hy; Su Jc 1992

• Authors: Hu hy; Chen Lh; Sung Hy; Su Jc
• Title: Molecular cloning of chalcone synthase cdna from nicotiana-tabacum by the polymerase chain reaction
• Location: Bulletin of taiwan tobacco research institute taiwan tobacco & wine monopoly bureau, 0 (37). 1992. 9-22.
• Abstract: sequence comparisons of previously identified chalcone synthase (chs) cdna from various plant species showed that some parts of the sequences have highly conserved. according to these conserved sequences, several 20-25 bases in length oligonucleotides were synthesized as primers. first strand cdnas were synthesized from total rna of tobacco flower as template. a partial chs cdna of 866 bp segment was identified by polymerase chain reaction (pcr) amplification followed by southern hybridization with 32p labeled parsley chs cdna probe. the dna sequence of the pcr product showed high homology with the chs genes of Antirrhinum majus, petunia hybrida, petroselinum hortense, phaseolus vulgaris, arabidopsis thaliana and sinapis alba l. the striking level of dna sequence homology between tobacco chs cdna and other plants confirms that the pcr product to be part of chs cdna.