Martin C et al. 1989a
- Authors:
Martin C. Prescott A. Lister C. MacKay S.
- Title:
Activity of the transposon Tam3 in Antirrhinum and tobacco: possible role
of DNA methylation.
- Reference location:
EMBO Journal. 8(4):997-1004, 1989 Apr.
- Abstract:
The transposon Tam3 from Antirrhinum majus can transpose in a heterologous
host (Nicotiana tabacum); thus the element is autonomous, probably
encoding the specific information required for its own transposition. In
transgenic tobacco Tam3 rapidly becomes methylated at its ends whilst
adjacent flanking sequences remain hypomethylated. This methylation may
account for our failure to detect Tam3 transposition in the progeny of
transgenic tobacco. Treatment with the inhibitor of cytosine methylation,
5 aza-cytosine appeared to induce transposon related activity at a low
level. In Antirrhinum methylation also appears to be associated with
inactivation of Tam3 copies.
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