Radunz A et al. 1975
- Authors:
Radunz A. Schmid GH.
- Title:
The effect of antibodies to neoxanthin on electron transport on the oxygen
evolving side of photosystem II and the reactions of this antiserum with
variuos chloroplast preparations.
- Reference location:
Zeitschrift fur Naturforschung - Section C - Biosciences. 30(5):622-7,
1975 Sep-Oct.
- Abstract:
Antibodies to neoxanthin agglutinate stroma-free swellable chloroplasts
from tobacco (Nicotiana tabacum var. John William's Broadleaf) and
Antirrhinum (Antirrhinum majus) whereas stroma-freed chloroplasts, which
have lost the swellability are not agglutinated despite the fact that
antibodies to neoxanthin are specifically absorbed. In this latter case
the agglutination is hindered for sterical reasons. From this it is
concluded that neoxanthin is located in the outer surface of the thylakoid
membrane. The antiserum to neoxanthin inhibits the ferricyanide
photo-reduction in chloroplasts when water is the electron donor by 15%.
With diphenylcarbazide in tris-treated chloroplasts no inhibition is
observed. Hence, just as in the case of the antiserum to lutein the site
of inhibition is on the donor side of potosystem II namely between water
and the site of electron donation of diphenylcarbazide.
Benzidine/ascorbate is another artificial electron donor system of
photosystem II reported in the literature. The photoreduction of
anthraquinone-2-sulphonate with this donor system is inhibited. In
contrast to the antiserum to lutein the antiserum to neoxanthin inhibits
DCMU-sensitive photophosphorylation reactions in the system H2O leads to
ferricyanide and benzidine/ascorbate leads to anthraquinone-2-sulphonate.
Therefore, the electron transport coupled to photophosphorylation is
inhibited by the antiserum.
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