Sommer H et al. 1988
- Authors:
Sommer H. Bonas U. Saedler H.
- Title:
Transposon-induced alterations in the promoter region affect transcription
of the chalcone synthase gene of Antirrhinum majus.
- Reference location:
Molecular & General Genetics. 211(1):49-55, 1988 Jan.
- Abstract:
Four solid-colour revertants were isolated from the highly variegated
niv-53::Tam1 mutant, in which the transposable element Tam1 is integrated
in the promoter region of the chalcone synthase (chs) gene. DNA sequence
analysis revealed that in all four lines the Tam1 element was deleted
together with flanking nucleotides of the chs promoter. In one case the
TATA box of the chs gene was removed resulting in extremely low expression
of the gene, and initiation of transcription occurring at a new position.
The other three deletions defined a sequence motif (TAC-CAT) which is
apparently required for maximal gene expression. Thus transposable
elements seem to be useful for probing gene structure, in this case the
signal structure in the promoter region, by virtue of imprecise excision.
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