Takasu-Ishikawa E et al. 1992
- Authors:
Takasu-Ishikawa E. Yoshihara M. Hotta Y.
- Title:
Extra sequences found at P element excision sites in Drosophila
melanogaster.
- Reference location:
Molecular & General Genetics. 232(1):17-23, 1992 Mar.
- Abstract:
We have previously established a transgenic Drosophila line with a highly
transposable P element insertion. Using this strain we analyzed
transposition and excision of the P element at the molecular level. We
examined sequences flanking the new insertion sites and those of the
remnants after excision. Our results on mobilization of the P element
demonstrate that target-site duplication at the original insertion site
does not play a role in forward excision and transposition. After P
element excision an 8 bp target-site duplication and part of the 31 bp
terminal inverted repeat (5-18 bp) remained in all the strains examined.
Moreover, in 11 out of 28 strains, extra sequences were found between the
two remaining inverted repeats. The double-strand gap repair model does
not explain the origin of these extra sequences. The mechanism creating
them may be similar to the hairpin model proposed for the transposon Tam
in Antirrhinum majus.
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